For all the hoopla over CRISPR/Cas9 and its mystical powers, the system still hasn’t passed the most basic test of any magic talisman – waving it over a locked gate while chanting “open sesame”! Fortunately, a band of scientists led by Amira Barkal and Richard Sherwood have now vindicated CRISPR’s powers, using it to open […]
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Search Results for: epigenetic editing
Manipulating DNA Methylation with dCas9-DNMT3A
Almost immediately after its inception as a biotechnological tool, CRIPSR/Cas9 showed that it was more than just a genome editor. Deactivated Cas9 (dCas9) is a precision RNA-guided DNA-binding domain with a growing range of effector domains that can be swapped out like LEGO. When it comes to epigenome editing with dCas9, histones have led the […]
LEXY: Optogenetic Nuclear Export for Proteins Looking to Go Places
Optogenetics is one of the flashier techniques of the past decade. Instead of slow chemical or blunt physical interventions, optogenetics controls events inside cells with the timing and precision of a beam of light. Optogenetic success stories thus far include light-activated neuron firing, heart beating, organelle positioning, protein dimerization, membrane localization, gene editing, and import […]
A TAD Closer to Understanding 3D Chromatin Structure
Interview with Dr. Jennifer Phillips-Cremins at Keystone Symposia’s Precision Genome Engineering and Synthetic Biology. My lab is really focused on understanding the epigenetic mechanisms that regulate early brain development. And the, sort of traditional way of studying epigenetic marks, is to zoom in and really focus on a particular modification at a precise location in […]
CRISPR Hack Enhances Stem Cell Differentiation
From horses in the farmyard to yeast in beer production, human beings have a long history of putting nature’s gifts to work. This can also be said of CRISPR technology, which utilizes a Cas9 DNA endonuclease and a guide RNA to target and destroy specific DNA sites. Nature had intended a role for CRISPR in bacterial […]