Some scientific breakthroughs are so transformative that there’s no containing their spread once they’ve seeded. By engineering cellular nuclear reactions with a biochemical twist, the most powerful deactivated Cas9 (dCas9) methyltransferase explodes from the lab of Tomasz Jurkowski at the University of Stuttgart. The free energy of this reaction forges not only a new tool […]
- "dnmt"
Search Results for: dnmt
Dnmt3C: A New Piece of DNA Methylation Machinery
A dog walk in the countryside led to Velcro, the search for chest pain therapies gave us (who me?) Viagra, and a messy microbiology lab bench provided penicillin. Some big research discoveries appear to have arrived in an “accidental” form and maybe, just maybe, new research from the laboratory of Déborah Bourc’his can be spoken […]
Manipulating DNA Methylation with dCas9-DNMT3A
Almost immediately after its inception as a biotechnological tool, CRIPSR/Cas9 showed that it was more than just a genome editor. Deactivated Cas9 (dCas9) is a precision RNA-guided DNA-binding domain with a growing range of effector domains that can be swapped out like LEGO. When it comes to epigenome editing with dCas9, histones have led the […]
DNMT1 Loss is Lethal in Human But Not Mouse Embryonic Stem Cells
Mouse models have been great to science, they’ve given insight into humans in ways simply not possible in humans. However, while there are striking similarities in the epigenetics of mice and men, there are also undoubtedly some fundamental differences. Now, a team from the Broad Institute of MIT and Harvard consisting of the labs of Alexander Meissner, J. Keith Joung, and John […]
DNMT1 Depletion Helps Hunt for Heritable Methylation
Sometimes it takes a special kind of guy to get the job done and when the job is to keep imprinted genes differentially methylated, that guy is DNMT1. An international group of researchers led by Jacquetta Trasler in Canada set out to exploit this fact in order to identify yet unknown imprinted genomic sequences. Several […]