Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event […]
- Millipore
CpG WIZ® Fragile X Amplification Kit
Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event […]
CpG WIZ® Amplification Kits
ools for Measuring Levels of Silencing-associated CpG Methlyation in DNA at Specific Genes Features Detect definitive changes in the CpG methylation pattern of genomic DNA Correlate transcriptional regulation with changes in methylation pattern Investigate epigenetic mechanisms of developmental control and disease progression Advantages Flexibility of experiment with a universal method for any gene of interest […]
CpG MethylQuest DNA Isolation Kit
The CpG MethylQuest DNA Isolation Kit allows for simple, effective and rapid enrichment of methylated DNA from genomic samples. Featuring a high affinity recombinant methyl-binding domain (MBD) protein pre-bound to magnetic beads, a rapid protocol, and a simple elution procedure that yields methylated DNA ready for downstream analysis, this high affinity kit enriches for methylated […]
CpGenome Fast DNA Modification Kit
The CpGenome Fast DNA Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, converting them to uracils, while 5-methylcytosines remain unaltered. Thus, the sequence of the treated DNA will differ depending on whether the DNA is originally methylated or […]