ChampionChIP qPCR Primers are available for study the proximal promoter region of every human, mouse, and rat gene. These primers are designed and validated for SYBR Green based real-time PCR. They are optimized to measure genomic DNA enrichment among ChIP-purified DNA samples. These primers are ready to use for studying transcriptional regulation of your favorite genes at or around their transcriptional start site (TSS). For example, transcription factor binding and histone modification around the TSS of your favorite genes can be reliably detected and quantified by ChIP-qPCR Primers. The enrichment or diminishment of a nuclear factor binding may be associated with a particular disease or developmental state in your cell system.
Transcription Factor Researchers:
- Use this search portal to find PCR primers for your favorite transcription factors\’ binding sites if you know their location relative to the TSS of your genes of interest.
- This search portal returns all 30 available primers tiled around the TSS of the queried gene.
- Use the drop-down button to select the tile closest to your binding site and see the relative position of the primers.
- Because primer designs fall anywhere within a specified 1-kb tile region, remember that the closest assay to your binding site may actually lie in an adjacent tile.
Histone Modification Researchers:
- Use this search portal to identify PCR primers for specific regions in your favorite gene bound by histones with specific modifications. For example:
- The primers for the +1 and -1 kb tiles are often used to define two major groups of histone modifications related to transcriptional activation and repression of most genes.
- The primers for all thirty tiles may be used in a Custom PCR Array to discover the binding positions of different histone modifications across your favorite gene\’s promoter.