Benefits of SABiosciences’ SYBR Green qPCR Master Mixes:
Accuracy: Guarantees single gene-specific products with minimal primer dimers.
Reliability: Maximizes amplification efficiencies.
Convenience: Specially formulated all-in-one mixes that match the instrument in your lab.
- RT2 SYBR Green/ ROX: For all ABI Instrumentation
- RT2 SYBR Green/ Fluorescein: For all BioRad Instrumentation
- RT2 SYBR Green: For instrumentation not requiring reference dye
Master Mix quality plays a critical role in the performance of SYBR Green-based real-time PCR. The key to obtaining accurate results is a tightly-controlled hot start enzyme that eliminates the amplification of primer dimers and other secondary products. Antibody-based technologies have worked well in the past for most PCR applications. However, these enzymes too often “leak” and synthesize too many non-specific amplicons to meet the more rigorous and demanding standards set by SYBR Green real-time detection. After years of experience in designing and experimentally testing thousands of PCR primers for entire genomes, SABiosciences has developed a qPCR master mix formulation that provides the most accurate SYBR Green real-time PCR results.
Unique HotStart Polymerase and Buffer Components
The RT2 qPCR Master Mixes from SABiosciences offer superior specificity and amplification efficiency thanks to our high-quality chemically-modified HotStart Taq DNA polymerase. Hot start enzymes found in other sources of master mix, such as the antibody-based technologies, still retain residual activity in their “inactive” state at low temperature. Under these conditions, non-specific priming events are extended to form templates which are then amplified into non-specific products that artificially contribute to the SYBR Green signal and cause false positive results. Our tightly controlled HotStart enzyme lacks any activity before being heated and therefore minimizes the amplification of non-specific PCR products.
The RT2 qPCR Master Mix formulations also include other proprietary chemical components that significantly minimize primer dimer formation ensuring high amplification efficiencies for even the most difficult to amplify genes.
All-in-One Master Mixes: Instrument Reference Dyes Included
Our RT2 qPCR master mixes are tailor-made and optimized for the specific real time system in your lab. These cost-effective all-in-one PCR master mixes not only contain SYBR Green, optimized buffer, nucleotides and our high-quality HotStart DNA polymerase. They also include the appropriate reference dye specifically used by individual real time systems to normalize their optics. Just add your primer pairs and templates, and you’re ready for PCR.
Complete your assays using the RT2 qPCR Primer Assays or the RT2 Profiler PCR Arrays with the appropriate RT2 SYBR Green qPCR Master Mix tailor-made to the instrument in your laboratory.View RT2 qPCR Master Mixes on SA Biosciences’s website