The RT2 miRNA PCR Array accurately analyzes the expression of multiple micro RNA sequences at the same time on ANY real-time PCR instrument. Each 96- or 384-well plate contains SYBR Green optimized and experimentally validated assays for either the entire human or mouse miRNA genome or pathway-focused sets of human or mouse miRNA sequences. Our proprietary miRNA detection technologies enable uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. These technologies also provide the RT2 miRNA PCR Array with high sensitivity, specificity and accuracy. Five miRNA arrays are currently available for both human and mouse.
Available miRNA PCR Arrays:
- Cancer
- Cell Differentiation & Development
- Whole Genome
- miFinder
Why miRNA PCR Arrays?
- Biologically Relevant: Arrays represent the entire human or mouse miRNA genome or a pathway- or disease-focused panel such as cancer and development.
- Reliable, Sensitive, Specific: Simple real-time PCR method with optimized primer design and reaction formulation improves discrimination and sensitivity.
- Simple & Easy to Use: Brings miRNA expression profiling to almost any lab with a real-time PCR instrument.
Simple Workflow
Simply mix cDNA template, generated from our first strand kit, with the appropriate ready-to-use PCR master mix. Then, aliquot equal volumes to each well of the same plate, and run the real-time PCR cycling program. PCR Arrays are compatible with all ABI, Bio-Rad, Stratagene and Roche instrumentation.
Well Designed Layout and Controls
The miRNA PCR Arrays are available in both 96- and 384-well plates to monitor the expression of 88 or 376 miRNA sequences either from the entire human or mouse genome or an application-focused panel. Four housekeeping sequences and other controls are also included on each array for data normalization, RNA quality and general PCR performance.
Easy-to-Use Data Analysis
Data analysis is based on the ddCt method with normalization of the raw data to one or more of the housekeeping assays.
You can easily perform a miRNA PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.
Performance
Discrimination & Specificity, Sensitivity & Dynamic Range, Reproducibility
Real-time RT-PCR is the most sensitive and reliable method for analyzing the expression of nucleic acids like miRNA. Its wide dynamic range makes it the preferred choice for simultaneously quantifying both rare and abundant sequences in the same sample. However, the small size and high degree of similarity among miRNA sequences makes quantitative analyses of their abundance very challenging. Any assay for miRNA must not only demonstrate the sensitivity and reproducibility expected of real-time PCR, but also be specific enough to discriminate between sequences that differ by as little as one mismatch. The experimental results below will show that the RT’ miRNA PCR Array and Assay System meets these performance criteria.
View RT2 miRNA PCR Array on SA Biosciences’s website