The traditional ChIP methods, based on agarose or magnetic beads, involve many steps of separation, pipetting and re-suspension. These sequences can either be done by magnetism or centrifugation, which are repetitive and prone to errors.
Chromatrap® is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. These kits use spin columns which contain discs of an inert, porous polymer to which protein A or protein G has been covalently attached. During an assay the target chromatin/antibody complex is retained by the disc, flushed with three buffers and eluted to obtain the DNA fragments of interest with the following benefits:
- Levels of DNA pull down up to 25 times higher than with bead based procedures.
- Excellent DNA enrichment with signal to noise ratios typically 2 to 3 times better than competing procedures.
- Can be used for a wide range of chromatin loadings depending on your source of materials.
- High sensitivity with small sample sizes; as little as 100ng (15,000 cells) per column.
- High loadings of up to 3000ng (4.5 million cells) per column can be used in preparation for ChIP-Seq.