The Methyl-Profiler DNA Methylation Enzyme Kit prepares your genomic DNA samples for analysis by real-time PCR primers and arrays for known and predicted methylated CpG islands by real-time PCR. Using the enzymes and buffer provided in the kit, four digests are performed to detect different methylated DNA fractions. The product of a mock digest contains all of the input genomic DNA. The product of the methylation-sensitive restriction enzyme (Enzyme A) digest contains hypermethylated DNA sequences, while the product of the methylation-dependent restriction enzyme (Enzyme B) digest contains unmethylated DNA sequences. The product of a double digest measures the background and the success of both enzymatic digestions. Use the DNA Methylation Enzyme Kit together with the Methyl-Profiler?DNA Methylation PCR Arrays and Custom qPCR Primers to analyze the methylation status of CpG islands in the promoter of your genes or other methylated DNA sequences of interest.
View DNA Methylation Enzyme Kit on SA Biosciences’s website- DNA Methylation Enzyme Kit