EpiTect HRM PCR Kit

For reliable screening of changes in methylation status by HRM analysis

• Accurate detection of changes in CpG methylation status
• Highly specific PCR amplification without the need for optimization
• Distinct melting curves due to EvaGreen fluorescent dye
• Convenient master mix format and optimized protocol
• Fast and easy development of new HRM methylation assays

The EpiTect HRM PCR Kit uses high-resolution melting (HRM) technology for fast screening and accurate detection of changes in the CpG methylation status of bisulfite-converted DNA. The optimized kit is available in a convenient master mix format and contains the novel double-stranded DNA-binding fluorescent dye EvaGreen, as well as an optimized HRM buffer, HotStarTaq Plus DNA Polymerase, and dNTPs. The kit is compatible with real-time instruments suitable for HRM analysis, particularly the Rotor-Gene Q, and also the Rotor-Gene 6000 and the LightCycler 480.

The EpiTect HRM PCR Kit provides a highly reliable and cost-effective method for methylation analysis using HRM technology and can be successfully used for methylation studies in epigenetic research. The optimized protocol means that information on the methylation status of CpG sites can be derived quickly and efficiently. This simplifies methylation assay development.