The EpiTect MethyLight PCR Kit and the EpiTect MethyLight PCR + ROX Vial Kit enable sensitive and reliable quantification for all TaqMan or dual-labeled probe based methods for discrimination of methylation sites. Quantitative MethyLight assays, consisting of two probes one methylation-specific, and the other nomethylation-specific, as well as semiquantitative MethyLight assays, can be used in a single real-time PCR reaction. An optimized buffer containing the synthetic Factor MP creates stringent primer annealing conditions leading to increased PCR specificity.
View EpiTect® MethyLight PCR Kit on QIAGEN’s website- EpiTect® MethyLight PCR Kit