EpiTect Plus Bisulfite Kits facilitate effective bisulfite conversion of DNAÿwhile minimizing DNA degradation due to the harsh temperature and chemical conditions of the bisulfite conversion reaction. These kits have the added advantage of not requiring DNA isolation prior to the conversion reaction. This streamlined workflow, from sample lysis to final elution ,minimizes sample loss to generate high yields of high-quality DNA.ÿ This high recovery and superior protection of converted DNA are critical factors in the sensitivity of downstream applications, making these kits an essential component of your methylation analysis workflow. Key features: -No DNA isolation required before conversion -Over 99% DNA conversion rateÿwith minimal degradation -Highly concentrated converted DNAÿin low elution volumes (down to 10 æl)ÿ -Integrated technologies that minimize DNA loss during lysis and protect DNA, resulting in high yields -Carefully optimized protocols for genomic DNA, whole blood, cell cultures, tissue samples, and FFPE tissue slices -High input range for reliable results from 1 ng ? 2 æg gDNA, 0.5?20 æl whole blood, 10?100,000 cells, or 1 FFPE tissue slice (10 æm thick) EpiTect Plus Bisulfite Kits are interconnected solutions, functioning as an integrated system for direct bisulfite conversion of DNA from a broad range of starting materials. However, each kit has unique features, addresses a specific sample type, and includes all buffers and reagents necessary to function independently. Use the EpiTect Plus DNA Kit for genomic DNA, the EpiTect Plus FFPE Bisulfite Kit for formalin-fixed, paraffin-embedded tissue slices, and the EpiTect Plus LyseAll for whole blood, cell cultures, and tissue samples.
View EpiTect Plus LyseAll Bisulfite Kit on QIAGEN’s website- EpiTect Plus LyseAll Bisulfite Kit