The Methyl-Profiler DNA Methylation PCR Arrays profile the methylation status of panel of 24 or 96 gene promoters whose hypermethylation has been reported in the literature the most frequently in a variety of experimental samples. Profiling your biological DNA samples with these arrays will correlate CpG island methylation status with biological phenotypes or clinical outcomes. The results also provide insights into the molecular mechanisms and biological pathways behind disease and development. With a simple restriction enzyme digestion and real-time PCR on your 96- or 384-well instrument, you can analyze the methylation status of 24 or 96 different genes with the DNA Methylation PCR Arrays.
List of PCR Arrays Available:
- Human Breast Cancer
- Human Stem Cell Transcription Factors
- Human Gastric Cancer
- Human Inflammatory Response
- Human Liver Cancer
- Human T Cell Activation
- Human Lung Cancer
- Human Cytokine Production
- Human Prostate Cancer
- Human Colon Cancer
How It Works
The Methyl-Profiler PCR Array System relies on the differential cleavage of target sequences by two different restriction endonucleases whose activities require either the presence or absence of methylated cytosines in their respective recognition sequences. As real-time PCR quantifies the relative amount of DNA remaining after each enzyme digestion, the methylation status of individual genes and the methylation profile across a gene panel are reliably and easily calculated. The high yield of DNA from the restriction digests and PCR amplification allow the analysis of smaller, more heterogeneous samples.
What It Offers:
- Guaranteed Performance – Ready-to-use for DNA methylation analysis
- Time & Cost Savings – Less than 30 min hands-on time to analyze 24 or 96 genes
- Easy Data Analysis – FREE Excel-based data analysis template available
You can easily perform a Methyl-Profiler experiment in your own laboratory using any 96-well or 384-well real-time PCR instrument that you have access to.
Or you can send your DNA samples to us and take advantage of our PCR Array Services.
Array Layout:
The PCR Arrays are available in both 96- and 384-well plate formats to analyze the methylation status of 24 or 96 genes related to a disease state, such as specific types of cancer, or development pathways related to specific cells or tissues. The signature panels of 24 genes are arranged on either 96-well or 384-well plates to simultaneously characterize all four restriction digests from either one or four different DNA samples, respectively. The more complete panels of 96 genes are arranged on 96- or 384-well plates to characterize all four restriction digests from one DNA sample either on four plates or all on the same plate, respectively.