miRZip anti-sense microRNAs are stably expressed RNAi hairpins that have anti-microRNA activity. These miRZip shRNAs produce short, single-stranded anti-microRNAs that competitively bind their endogenous microRNA target and inhibit its function. The result is the derepression and elevation of the protein levels of the transcripts targeted by the microRNA being “zipped”.
The miRZip short hairpin RNAs are cloned into SBI’s pGreenPuro shRNA expression lentivector. The miRZip hairpins are rationally designed for asymmetry such that the upper strand of the hairpin does not contain the endogenous microRNA sequence and the lower strand is preferred for producing anti-sense microRNAs that are fully complementary to a specific microRNA target.
Thoroughly Understand MicroRNA Function
SBI’s microRNA interference Lentivector System provides a convenient and effective approach to create stable cell lines or transgenic animals since it efficiently integrates the anti-microRNA expression construct of your choice into genomic DNA. Assays with cell lines or transgenic animals that permanently and heritably maintain a microRNA-specific suppression phenotype enable you to more thoroughly analyze the specific effects resulting from inhibiting your target microRNA.
Unique, Highly Effective System
Our FIV and HIV-based Cloning and Expression Lentivectors are derived from the next generation of self-inactivating lentiviral vectors. This unique system offers a highly effective and very safe approach for introducing and expressing any anti-microRNA sequence in nearly any mammalian cell system.
Single Promoter Format
The pGreenPuro siRNA/anti-microRNA lentivector employs a CMV promoter for the expression of dual copGFP and Puromycin markers and an H1 promoter for the expression of high levels of anti-microRNAs in the target cell.
Complete Lentivector Packaging System
In order to stably transduce cells with SBI’s lentivectors, we recommend using SBI’s pPACK-H1 Lentivector Packaging System (cat.#LV500A-1) together with SBI’s 293TN cell line (cat.#LV900A-1). The packaging system provides all the necessary coat proteins to manufacture pseudoviral particles for delivery of the anti-microRNA expression construct into any mammalian cells or model organisms, such as transgenic mice.
Features and Benefits
- Stable & permanent anti-microRNA expression from constitutive H1 promoter
- Rationally designed, asymmetric hairpins optimized for anti-sense microRNA production
- miRZip anti-sense microRNAs efficiently suppress specific endogenous microRNAs
- Reliable delivery to dividing or non-dividing cells
- Select for positive expressing cells with either GFP or Puro selection
- Uncover phenotypes using powerful anti-microRNA interference
- Pool of prepackaged viral particles containing the entire anti-microRNA collection for high-throughput phenotypic screens also available