Active Motif’s new MODified™ Histone Peptide Arrays (Catalog Nos. 13001 & 13005) provide researchers with a valuable tool to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications. The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide. This extensive coverage of histone modifications enables the study not only of individual sites, but also the effects of neighboring modifications on recognition and binding. The simple array protocol works like a Western blot; no special equipment needed.
To assist in the analysis, Active Motif has launched a FREE software program designed for use with the MODified Histone Peptide Arrays. The software program will analyze spot intensities on the array and generate a graphical analysis of the histone peptide modification interactions. Information about spot intensity, averages and errors can be saved in Excel-compatible files. Additionally, up to three individual histone modifications can be displayed in superposition to the experimental data, enabling better visualization of the effects of neighboring modifications.View MODified™ Histone Peptide Arrays on Active Motif’s website