PyroMark CpG Assays are predesigned assays that enable Pyrosequencing analysis of methylation at specific targets across the human and mouse genomes. Generated with carefully tailored algorithms for optimal assay design based on bisulfite converted DNA, these fast and easy to order assays minimize time spent on assay optimization and maximize analysis success. Currently, our database of assays includes approximately 60,000 assays covering almost 80% of gene-specific islands in the human and mouse genomes. Assays are continuously updated and improved, and the database is being expanded to include other organisms. Key features: – Approximately 60,000 individual assays for human and mouse – Coverage of almost 80% of gene-specific CpG islands – Advanced design algorithms for optimized results – Convenient download of run and analysis settings – Top performance from a cost-effective solution – Available in tube and 96-well plate format Primers included in PyroMark CpG Assays are designed by rigorous algorithms and checked against the entire bisulfitome to ensure specificity to deliver PCR and sequencing primers at optimal concentrations for high PCR specificity. Together with the carefully formulated reagents of other dedicated products of the PyroMark line, this optimization ensures improved yield and quality of sequencing template, which conversely increases assay reliability.
View PyroMark CpG Assay 96 Well on QIAGEN’s website- PyroMark CpG Assay 96 Well