RT2 qPCR-Grade miRNA Isolation Kit

Using total RNA in real-time PCR assays for miRNA performs well with some sequences and some cell lines and tissues. For most other miRNA sequences and biological material, the presence of the other RNA species besides miRNA amplifies secondary products, increases the non-specific background, and diminishes sensitivity and dynamic range. The RT² qPCR-Grade miRNA Isolation Kit brings miRNA profiling to wider variety of labs and research interests by specifically isolating small RNA enriched for miRNA. The exclusive use of small RNA to profile miRNA eliminates the amplification of non-specific products from irrelevant RNA. The RT² qPCR-Grade miRNA Isolation Kit helps maintain the specificity and sensitivity expected of real-time PCR for all miRNA sequences in many more types of samples.

Benefits of the RT² qPCR-Grade miRNA Isolation Kit:

• High Quality and High Yield: Isolate enough high-quality miRNA from cells and tissues with a protocol optimized for real-time RT-PCR expression analysis
• Reliable and Sensitive: Find more miRNA sequenes expressed at lower levels while eliminating false positives
• Fast and Convenient: Go from as many as 12 samples to purified miRNA with less than 60 minutes of hands-on time

Better RNA quality means better real-time RT-PCR results.

Micro RNA quality is one of the most important factors for successful expression analysis by real-time RT-PCR. SABiosciences has years of experience helping researchers who are new to expression analysis technologies and who wish to use a wide variety of sample sources. We know what it takes to get miRNA of sufficient quantity and quality for real-time PCR experiments. SABiosciences has put this level of expertise into its RT² qPCR-Grade miRNA Isolation Kit for researchers to take advantage of our experience and perform their experiments right the first time.

HOW IT WORKS:

SABiosciences’ RT² qPCR-Grade miRNA Isolation Kit is optimized for real-time RT-PCR based gene expression analysis. It is designed to extract up to 10 µg of miRNA that is free of genomic DNA, large RNA, and other impurities. The protocol works in combination with a phenol/chloroform-based extraction method for cultured cells and animal tissues.

The special silica membrane spin column technology, with appropriate ethanol concentrations, permits small RNA to flow-through while longer RNA and DNA bind an initial column. Re-adjusting the ethanol concentration in this eluate then permits optimal retention of the miRNA on a second equivalent column. Washing the second column removes salts, metabolites, and other macromolecular cellular components. Low ionic strength conditions finally elute pure miRNA ready for subsequent cDNA template synthesis with the RT² miRNA First Strand Kit (MA-03).

The RT² qPCR-Grade miRNA Isolation Kit provides an easy and efficient approach to acquire miRNA of a greater purity and higher yield. The protocol takes only 60 minutes of hands-on time, and the kit includes sufficient materials to purify 12 RNA samples. RNA purified by this kit has been thoroughly tested on and optimized for our RT2 miRNA qPCR System.