One of the biggest challenges associated with preparing high quality ChIP-Seq libraries is sample loss. Traditional library preparation methods require multiple end-repair and ligation steps which contribute to loss of material. To overcome this challenge, Active Motif has introduced its TAM-ChIP technology to perform chromatin immunoprecipitation and next-generation library preparation at the same time.
TAM-ChIP utilizes the specificity of a ChIP-Seq validated antibody to target protein binding sites of interest and a TAM-ChIP anti-species antibody conjugate in combination with a unique TsTn5 transposase to deliver barcoded sequencing adapters directly at the target site. Following immunoprecipitation, enriched DNA is ready for library amplification and sequencing on Illumina®-compatible platforms. This streamlined process minimizes sample loss for more reliable and robust ChIP-Seq results.View TAM-ChIP on Active Motif’s website