ChIP 101: Beginner’s Guide to ChIP AssaysJuly 24, 2019Become a ChIP Assay Expert! The chromatin immunoprecipitation (ChIP) assay is one of the best ways to study the association of DNA-binding proteins with chromatin and the localization of histone modifications throughout the genome. But ChIP assays take time to master. Our friends at Active Motif have put together this free ChIP 101 eBook that […]
eBook: NeuroepigeneticsJuly 4, 2019It’s become increasing clear that epigenetic mechanisms have a unique role to play in the brain. From neurodevelopment, to memory, to brain function epigenetic mechanisms seem to be very important. These mechanisms are also frequently perturbed in neurological disorders. In this ebook, we’ve assembled summaries of research related to uncovering the many roles of epigenetics […]
eBook: Cancer and EpigeneticsMay 15, 2019Changes in epigenetic regulation are hallmarks of the transition from normal to cancerous cells. Exploration of the role of epigenetics in cancer has proven to be informative for better understanding both cancer and epigenetic mechanisms. In this EpiGenie ebook, we present summaries of several studies on exploring the role of specific epigenetic processes in cancer. […]
eBook: Aging and EpigeneticsJune 8, 2018This eBook explores various levels at which aging and epigenetics are related. Assembled are summaries of studies exploring specific questions regarding the epigenetic basis of aging and aging-related conditions. They are divided into four chapters: 1. DNA Methylation Epigenetic Clock 2. Anti-aging 3. Alzheimer’s Disease 4. Stem Cells
eBook: Key Chromatin Players | H3K27December 6, 2017Histone H3 lysine 27 (H3K27) is one of the most studied histone modifications with a complex biological role. Trimethyaltion of K27 is a hallmark of repressed transcription, while acetylation is associated with active transcription. Recent work has supported these roles as well as provided evidence for novel functions. This ebook covers the basics of H3K27 modifications, their role […]
eBook: Key Chromatin Players | H3K4June 24, 2017Histone H3 lysine 4 (H3K4) is one of the most widely studied histone modifications. Acetylation (H3K4me) and various degrees of methylation (H3K4me) of this residue are involved in countless processes. In general, H3K4 modifications are associated with active transcription. Recent research has further characterized these roles as well as identified new functions. This ebook covers […]
eBook: ChIP Seq Data Analysis Tools & DatabasesSeptember 13, 2016Chromatin immunoprecipitation (ChIP) is a powerful workflow for analysis of chromatin structure. The antibody-based pulled down of protein-DNA interactions is only half the process. Once the genomic regions bound by a protein are enriched and sequenced, bioinformatics tools and pipelines are necessary to identify, characterize, and compare these regions between samples. This ‘dry’ side of the ChIP workflow is […]
eBook: ChIP Reloaded | Recent Advances & Technical GuidanceMarch 25, 2016In this eBook, we’ve summarized several recent technical advances in ChIP that simplify the process and improve performance. Then we tossed in invaluable technical guidance from ChIP experts tackling every thing from working with challenging samples to data normalization. To top it all off, we’ve highlighted some new web applications that aim to streamline ChIP data analysis […]
eBook: DNA Demethylation in DevelopmentMarch 19, 2016This ebook provides an introduction to the key cytosine base modifications: 5-methylcytosine, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) that have been at the center of intense study in recent years. Inside, we’ve compiled numerous highlights that provide background on how these modifications function in DNA demethylation and also highlight the growing data that captures the role of demethylation […]
Guide: Optimizing ChIP Seq for FFPE SamplesFebruary 25, 2016Inside the world’s formalin-fixed, paraffin-embedded (FFPE) tissue repositories exists an enormous store of biological information. This valuable preservation technique allows researchers to perform histological and even genetic analysis years after sample collection. Although the FFPE process is a valuable preservation process, it presents challenges to researchers seeking to apply epigenetic techniques such as chromatin immunoprecipitation […]
