Chromatin analysis experiments can be a roller coaster of emotion. One moment you’re flying high and the next you’re in a troubleshooting inferno. Fortunately, there has been quite a bit of competition to fine-tune nearly every portion of the ChIP sequencing workflow over the last few years, and those advancements are now benefitting researchers like […]
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Search Results for: ChIP
eBook: ChIP Reloaded | Recent Advances & Technical Guidance
In this eBook, we’ve summarized several recent technical advances in ChIP that simplify the process and improve performance. Then we tossed in invaluable technical guidance from ChIP experts tackling every thing from working with challenging samples to data normalization. To top it all off, we’ve highlighted some new web applications that aim to streamline ChIP data analysis […]
enChIP Kit
The enChIP Kit (engineered DNA-binding molecule-mediated chromatin immunoprecipitation) utilizes the CRISPR/Cas system to target specific genomic regions for investigation of associated DNA-binding interactions. A guide RNA (gRNA) containing 20 nucleotides complementary to the desired genomic region is expressed in combination with a tagged, enzymatically inactive Cas9 protein (dCas9). The gRNA directs the dCas9 protein to the […]
Guide: Optimizing ChIP Seq for FFPE Samples
Inside the world’s formalin-fixed, paraffin-embedded (FFPE) tissue repositories exists an enormous store of biological information. This valuable preservation technique allows researchers to perform histological and even genetic analysis years after sample collection. Although the FFPE process is a valuable preservation process, it presents challenges to researchers seeking to apply epigenetic techniques such as chromatin immunoprecipitation […]
LobChIP: A Single Day ChIP-Seq Library Preparation Hack
With the speed and power of today’s high-throughput sequencers it seems that chromatin immunoprecipitation is the ‘limiting reagent’ in your ChIP-Seq reaction. Usually ChIP-Seq immunoprecipitation and library preparation takes 4 or 5 days, but now a talented team from Uppsala University in Sweden bring forth the library-on-beads ChIP (lobChIP) protocol, which wraps that up into […]