ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with non-specifically captured chromatin. During PCR analysis, this contaminating DNA can lead to false positive PCR products, making data interpretation difficult. To help, Active Motif offers a variety of control options to help […]
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UnMethylCollector is a novel technology that allows for positive identification ofunmethylated DNA fragments. The kit specifically enriches for unmethylated CpG islands using a CXXC binding domain from mouse Mbd1. UnMethylCollector allows researchers to identify hypomethylated promoters from normal or diseased samples. This simple and sensitive technique provides DNA suitable for use in many downstream applications, such as real time […]
Active Motif’s MethylDetector™ Bisulfite Modification Kit simplifies analysis of DNA methylation. It comes complete with optimized reagents for performing DNA conversion with bisulfite, plus time-saving DNA purification columns and positive control PCR primers to validate your results.
Whereas a large body of evidence exists regarding the existing form of methylation, very little is yet known about 5-hydroxymethylcytosine methylation. 5-methylcytosine is converted into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases, but little is known about the role of 5-hydroxymethylcytosine in the cell. Many tools exist to study 5-methylcytosine, but few are able […]