The ChIP-IT® High Sensitivity (HS) Kit is designed to give superior performance when using antibodies against low abundance transcription factors, antibodies with sub-optimal binding affinities or when starting with a limited number of cells. Higher signal and lower non-specific DNA pulldown is achieved through the incorporation of specialized protein G agarose beads and optimized fixation, […]
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NOMe-Seq Kit
NOMe-Seq is a first of its kind kit to simultaneously analyze nucleosome occupancy and CpG methylation status on the same DNA strand. NOMe-Seq (Nucleosome Occupancy and Methylome sequencing) is a published method that was developed by the Peter Jones lab at USC and has been validated for gene-specific analysis using both traditional cloning and Sanger […]
MethylCollector™ Ultra
Active Motif’s MethylCollector™ Ultra offers a fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides. Enriched DNA can be used in many downstream applications, such as […]
Hydroxymethyl Collector
The Hydroxymethyl Collector™ Kit is designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation. The Hydroxymethyl Collector kit utilizes a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin […]
5-Carboxylcytosine (5-caC) antibody
5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]