ChIP-IT Express improves on traditional ChIP by reducing or eliminating several time-consuming steps. The kit’s magnetic beads have much lower background so pre-clearing and blocking are no longer necessary. Washing is easier because the spin steps have been replaced by rapid magnetic pull-down. Only 3 washes are required, as compared to 8 washes with traditional […]
- Active Motif
ChIP-IT Express Kits utilize magnetic protein G-coated beads, which improves on traditional ChIP by reducing or eliminating several time-consuming steps. The magnetic beads have much lower background than traditional agarose beads, so steps like pre-clearing and blocking are no longer necessary. Lower background also makes it possible to discern effects that are smaller in magnitude, and […]
ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with non-specifically captured chromatin. During PCR analysis, this contaminating DNA can lead to false positive PCR products, making data interpretation difficult. To help, Active Motif offers a variety of control options to help […]
UnMethylCollector is a novel technology that allows for positive identification ofunmethylated DNA fragments. The kit specifically enriches for unmethylated CpG islands using a CXXC binding domain from mouse Mbd1. UnMethylCollector allows researchers to identify hypomethylated promoters from normal or diseased samples. This simple and sensitive technique provides DNA suitable for use in many downstream applications, such as real time […]
Active Motif’s MethylDetector™ Bisulfite Modification Kit simplifies analysis of DNA methylation. It comes complete with optimized reagents for performing DNA conversion with bisulfite, plus time-saving DNA purification columns and positive control PCR primers to validate your results.