5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]
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MODified™ Histone Peptide Arrays
Active Motif’s new MODified™ Histone Peptide Arrays (Catalog Nos. 13001 & 13005) provide researchers with a valuable tool to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications. The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation and citrullination modifications on the N-terminal tails of histones H2A, […]
ChIP-IT™ Express HT
ChIP-IT Express improves on traditional ChIP by reducing or eliminating several time-consuming steps. The kit’s magnetic beads have much lower background so pre-clearing and blocking are no longer necessary. Washing is easier because the spin steps have been replaced by rapid magnetic pull-down. Only 3 washes are required, as compared to 8 washes with traditional […]
ChIP-IT™ Express Enzymatic
ChIP-IT Express Kits utilize magnetic protein G-coated beads, which improves on traditional ChIP by reducing or eliminating several time-consuming steps. The magnetic beads have much lower background than traditional agarose beads, so steps like pre-clearing and blocking are no longer necessary. Lower background also makes it possible to discern effects that are smaller in magnitude, and […]
ChIP-IT™ Control Kit – Human
ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with non-specifically captured chromatin. During PCR analysis, this contaminating DNA can lead to false positive PCR products, making data interpretation difficult. To help, Active Motif offers a variety of control options to help […]