The AbSurance Complete Core Histone Antibody Specificity Array is comprised of 89 high quality modified and unmodified histone peptides ( >95 % purity) spotted on Immobilon® P PVDF membranes in a 96-well format. One membrane contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified). The other membrane contains 43 histone H2A, H2B, H4 peptides (13 acetylated, 16 methylated, 6 phosphorylated, and 8 unmodified). Both membranes in the AbSurance Complete Core Histone Antibody Specificity Array kit contain 100 ng and 10 ng quantities of each peptide, in addition to 10 ng each of positive control IgG from rat, mouse, sheep, and rabbit. The control IgG, and a cut corner at the A1 position of each membrane, can be used as location reference marks for easy identification of spots. Peptides are evenly distributed on the array to enable quantification of distinct spots, and accurate determination of cross-reactivity. The format of the AbSurance Complete Core Histone Antibody Specificity Array enables a Western-blot–like detection approach using standard chemiluminescent reagents followed by image capture with either film or a CCD imaging system.
Features and Benefits
- Simple Western blot-like detection procedure
- High quality ( >95 % purity) peptides analyzed by mass spectrometry
- A total of 89 peptides on two membranes, for easy screening of histone H3, or H2A, H2B, and H4 peptides
- Two concentrations (10 ng and 100 ng) of each peptide allows detection of both strong and weak cross-reactivity
- Consistent and uniform spotting of peptides using proprietary process
- Data collection using either film or standard CCD imaging systems
- Easy- to-read 8 x 12 macroarray – no special imaging equipment or software required
- Positive control IgG spots and membrane landmarks for simple and easy spot identification