Active Motif’s new ChIP-Bisulfite-Sequencing (ChIP-Bis-Seq) Kit offers a highly optimized and user-friendly method to directly interrogate both the chromatin and DNA methylation profile on the same sample within a single experiment. The kit combines a chromatin immunoprecipitation (ChIP) procedure for target-specific enrichment of protein-bound or modified chromatin with bisulfite conversion and sequencing (Bis-Seq) to provide a DNA methylation profile at single nucleotide resolution of ChIP-enriched DNA. The ChIP-Bis-Seq Kit provides researchers a user-friendly method to perform genome-wide studies of the interplay between DNA methylation and chromatin features, such as in assessing allele-specific DNA methylation variances (e.g. imprinting, X-inactivation), or in evaluating global gene regulatory effects.
The ChIP-Bis-Seq Kit consists of three modules:
- The first is Active Motif’s ChIP-IT® High Sensitivity Kit which is used to immunoprecipitate chromatin bound to a protein or modification of interest in order to identify DNA binding sequences. Our ChIP-IT High Sensitivity assay includes all necessary buffers and reagents for chromatin preparation from cells or tissues, sonication to fragment the chromatin, and to perform up to 16 chromatin immunoprecipitation reactions.
- The second kit module, the Library Preparation Reagents, contains enzymes and methylated adapters compatible with Illumina sequencing platforms necessary to prepare 10 Next-Generation Sequencing (NGS) libraries from the ChIP-enriched DNA.
- The third kit module contains reagents to perform bisulfite conversion on the adapter-ligated DNA to determine the methylation status of each DNA strand. It includes sodium bisulfite conversion reagents with specialized DNA purification columns suitable for use with converted, fragmented ChIP-enriched DNA.