ChIP-IT® ChIP-Seq

The combination of ChIP with genome-wide analysis using Next-Generation sequencing (ChIP-Seq) is a powerful approach that can provide insights into gene regulation by identifying global binding sites for proteins of interest. Active Motif’s ChIP-IT^® ChIP-Seq Kit provides proven reagents, streamlined protocols and validation controls to give you confidence in successful ChIP-Seq using the Illumina^®sequencing platforms. The assay utilizes the highly consistent and robust chromatin immunoprecipitation and purification procedure of the ChIP-IT^® High Sensitivity Kit, includes Active Motif’s ChIP-IT^® qPCR Analysis Kit for ChIP DNA validation and also contains a set of library construction reagents suitable for the preparation of ten Next-Gen sequencing libraries.

ChIP-IT^® ChIP-Seq Method

ChIP-Seq is a multi-step process that includes chromatin preparation, immunoprecipitation, DNA purification, ChIP DNA validation and sequencing library construction. The procedure requires multiple days to complete, therefore it is strongly advised to plan your experiments in advance. The ChIP-IT^® ChIP-Seq kit provides validated reagents to perform 16 chromatin preparation and immunoprecipitation reactions, 10 standard curves for ChIP DNA validation and 10 sequencing library constructions for use with the Illumina sequencing platforms. We recommend using 30 µg chromatin (4.5 million cell equivalents) per chromatin immunoprecipitation to ensure sufficient ChIP DNA for library construction.