The LowCell ChIP kit ensures the use of low amount of reagents per reaction (not only cells but also antibodies, inhibitors, buffers). The kit also includes fewer buffers in comparison with other kits. This makes this kit cheaper to use and much simpler to work with. The number of steps is reduced and handling is easier with our LowCell Magnetic procedure. Moreover, our Magnetic Rack together with our new LowCell Magnetic ChIP protocol ensures the best IP conditions by working at a constantly cooler temperature.
The Diagenode Magnetic Rack has been designed to be used in IP experiments, keeping samples cool longer and allowing the use of small tubes to reduce the reaction volumes and waste of reagents. The kit protocol has been validated with our previous Shearing kits, and Bioruptor. Nevertheless, chromatin can be sheared with any in house protocol and sonication apparatus as long as efficiency is checked before use (see the Important note section: compatibility between protocols and kits).
The Diagenode LowCell ChIP kit contains protein A-coated paramagnetic beads (and negative IgG from rabbit) to allow you to work with rabbit polyclonal antibodies. As positive control or target antibody, choose one of our ChIPgrade rabbit polyclonal antibodies against main histone modifications or your protein of interest. If performing ChIPwith monoclonal antibodies, we also offer the negative IgG from mouse and protein G coated paramagnetic beads. For positive ChIP controls, see our list of ChIP grade monoclonal antibodies.View LowCell# ChIP kit on Diagenode’s website