Perform Automated MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. Diagenode’s Auto MeDIP kit has been optimized on the SX-8G IP-StarTM Automated System enabling highly reproducible results and allowing for high throughput assays.
- Generate highly consistent results with internal controls in 24h
- Minimize error with many reagents in 1 tube
- Optimized purification and de-crosslinking step (DIB – DNA isolation buffer)
- Allows direct correlation between IP’d material & methylation status
- Validated on SX-8G IP-Star Automated System
The combination of this High Quality Kit and the SX-8G IP-Star will allow you to perform MeDIP in less than 10 hours! Starting with sheared chromatin the Automated System will provide you with the purified imunoprecipitated DNA of your sample. The Auto MeDIP kit protocol has been validated using DNA sheared by sonication using the Bioruptor®. The Kit includes a DNA isolation buffer (DIB) for an extra-fast method to purify your IP’d material (for qPCR analysis). Alternatively, for other applications, e.g. sequencing, linear amplification or microarray, magnetic DNA purification (IPure) can be performed alternatively.View Auto MeDIP kit on Diagenode’s website