The Novel Diagenode MagMeDIP kit is designed to immunoprecipitate methylated DNA (Methyl DNA IP) this version is magnetic and further optimized and therefore presents a number of additional advantages. MagMeDIP kits uses, our antibody directed against 5-methyl Cytidine is provided as well as meDNA and unDNA internal IP controls.
The IP has been optimized to specifically select and precipitate the methylated DNA: by the use of our antibody, buffers and protocol. The IP efficiency can indeed be double-checked with the use of our internal controls. The MeDIP kits allow you to perform DNA methylation analysis of your sample together with optimized internal IP controls: ALL IN ONE TUBE.
This Methyl DNA IP method provides you with methylated DNA (meDNA) and unmethylated DNA (unDNA) controls to be used together with your DNA sample allowing direct CORRELATION between IPd MATERIAL and METHYLATION STATUS. This methylation analysis is FAST, HIGHLY SPECIFIC and each IP is QUALITY controlled: essential keys for RELIABLE results.
In the MagMeDIP kit, the protocol has been improved to allow researchers to work in smaller tubes than traditionally did so far. The kit ensures the use of low amount of reagents per reaction (not only antibodies, but also buffers). The kit also includes fewer buffers in comparison with other kits. This makes this kit cheaper to use and much simpler to work with.
The number of steps is reduced and handling is easier with our Magnetic Methyl DNA IP procedure. Modifications and Improvements have been listed in the Table below. Two MagMeDIP kit formats are available: one for a minimum of 10 IPs and another for a minimum of 32 IPs. In addition, the flexibility of the protocol has also been increased, as the IPd DNA can be isolated and/or purified in different ways based on the downstream application: an extra-fast and simplified protocol is included in the MagMeDIP kit (for qPCR analysis); but more traditional methods are also proposed (see option #2 and available module). Moreover, our Magnetic Rack together with our new MagMeDIP kit protocol ensures the best IP conditions by working at a constantly cooler temperature.View MagMeDIP Kit on Diagenode’s website