This 50 minute presentation covers the basic concepts of ChIP and advances in technology, including genome-wide profiling. You’ll learn approaches to assay design, performance, and data analysis to help avoid the most common and some uncommon experimental pitfalls. Chromatin Biology Research and Development, EMD Millipore Presenter: John M. Rosenfeld, Ph.D. Format: Audio with Synchronized Slides, […]
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Behind the Scenes Histone and DNA Methylation Regulation Tricks Revealed
Diehard fans cinema aren’t satisfied by just watching a movie; they want to know everything about it, including how those amazing special effects are pulled off. In much the same way, scientists at the University of Bergen in Norway noticed extensive gene expression reprogramming in their experiments with pre-cancerous prostate cells and wanted to get […]
DNA Methylation Affinity Methods Come With Some Baggage
If you sometimes wonder about the quality of data coming from today’s popular affinity-based DNA methylation analysis methods, you‘re not alone. In fact, researchers at Garvan Institute of Medical Research in Sydney Australia decided to do something about it by studying the techniques and learning that methylated DNA immunoprecipitation (MeDIP) and methyl-CpG binding domain-based capture […]
Do Transcription Factors Occupy All their Binding Motifs in the Genome? Not quite.
A British team of researchers recently decided to take closer look at the role of DNA methylation in conserved consensus motifs. Dr. Roger Foo from the Department of Medicine, University of Cambridge was nice enough to share a bit about their approach that recently published in BMC Genomics (Sept 2010) last week. Transcription factors recognise […]
Silencing of Ultraconserved ncRNAs Is Not So Golden in Cancer
If you live near a construction site, are listening to one half of a cell-phone conversation in a movie theater, or stuck on an airliner next to a fussy infant, then for you silence may indeed be golden. But, for certain ultraconserved non-coding RNAs, transcriptional silencing can lead to a cellular uproar. A new article […]