Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms. Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR […]
- Zymo Research
EpiQuest™ Genome-Wide DNA Methylation Analysis-Basic
The EpiQuest™ Genome-Wide DNA Methylation Analysis Platform combines Next-Gen sequencing with our well-established bisulfite technologies for the most comprehensive DNA methylation analysis service available. Streamlined sample prep, cutting-edge bioinformatics, and consolidated data delivery take the difficulty out of methylation characterization and simplify epigenetic biomarker discovery. Services Performed Genomic DNA Sample Validation Sample Processing and Library […]
Methylated-DNA IP Kit
The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific anti-5-methylcytosine monoclonal antibody for the “capture” and separation of methylated DNA from non-methylated DNA in only a few hours. Typically, over a hundred-fold […]
GpC Methylase -M.CviPI
For complete in vitro methylation of DNA for methylation analysis. Methylation of chromatin DNA for DNA accessibility studies. Inhibition of endonucleases with overlapping GpC sequence recognition. [3H]-labeling of DNA. The GpC Methylase (EC 2.1.1.37)1 from Zymo Research completely methylates all cytosine residues (C5) in double-stranded, nonmethylated and hemi-methylated DNA having the dinucleotide sequence 5?…GpC…3?. The […]
CpG Methylase – M.SssI
For complete in vitro methylation of DNA for methylation analysis. Methylation of chromatin DNA for DNA accessibility studies. Inhibition of endonucleases with overlapping CpG sequence recognition. [3H]-labeling of DNA. The CpG Methylase (EC 2.1.1.37) from Zymo Research completely methylates all cytosine residues (C5) in double-stranded, non-methylated and hemi-methylated DNA having the dinucleotide sequence 5’…CpG…3′. The […]