Active Motif’s RNA Subcellular Isolation Kit is designed to efficiently isolate separate nuclear and cytoplasmic fractions of RNA for downstream analysis. This method can be used to isolate both long and short RNA sequences from cells or tissue without cross-contamination or the use of phenolic compounds. RNA molecules exist at different levels of maturation and […]
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enChIP Kit
The enChIP Kit (engineered DNA-binding molecule-mediated chromatin immunoprecipitation) utilizes the CRISPR/Cas system to target specific genomic regions for investigation of associated DNA-binding interactions. A guide RNA (gRNA) containing 20 nucleotides complementary to the desired genomic region is expressed in combination with a tagged, enzymatically inactive Cas9 protein (dCas9). The gRNA directs the dCas9 protein to the […]
Global 5-hmC Quantification Kit
Active Motif’s Global 5-hmC Quantification Kit is an ELISA-based assay designed to detect and quantify 5-hydroxymethylcytosine (5-hmC) levels in genomic DNA from different sample types (e.g. normal and diseased), treatment conditions, clinical outcomes or environmental backgrounds. The Global 5-hmC Quantification Kit enables higher throughput processing of samples, requires only nanogram amounts of genomic DNA per […]
LightSwitch™ lncRNA Promoter Products
Long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of both transcription and chromatin organization. But, the promoters of lncRNA genes appear to be regulated differently than those of protein-coding genes. The LightSwitch Luciferase Assay System makes it fast & easy to study lncRNA promoter regulation using the same materials […]
Tag-ChIP-IT® Kit
The Tag-ChIP-IT® Kit offers a method to perform chromatin immunoprecipitation (ChIP) on transcription factor sequence variants, mutations and truncations without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag. Active Motif’s unique AM-tag sequence is specifically designed to work in ChIP. It has minimal […]