Whether you are performing drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications, substrate selection is critical. Nucleosomes offer the advantage of providing a more biologically relevant substrate when compared to histone proteins alone or synthetic peptides. To best mimic cellular biology with your in vitro assay, use Active Motif’s Recombinant Nucleosomes. […]
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ChIP-Bis-Seq
Active Motif’s new ChIP-Bisulfite-Sequencing (ChIP-Bis-Seq) Kit offers a highly optimized and user-friendly method to directly interrogate both the chromatin and DNA methylation profile on the same sample within a single experiment. The kit combines a chromatin immunoprecipitation (ChIP) procedure for target-specific enrichment of protein-bound or modified chromatin with bisulfite conversion and sequencing (Bis-Seq) to provide […]
ChIP-exo Kit
Active Motif’s ChIP-exonuclease (ChIP-exo) Kit offers an optimized and streamlined protocol for high-resolution genome-wide mapping of transcription factor binding sites in the human and mouse genomes. The fragmentation step of traditional ChIP protocols can produce oversized DNA fragments that interact with more than just the binding site of interest. ChIP-exo is a modified ChIP-Seq approach […]
Global DNA Methylation Assay – LINE-1
Global DNA Methylation Assay to quantify genome-wide 5-mC levels in human DNA Simple ELISA assay in a flexible strip-well microplate format Detects as low as 0.5% methylation Detects methylation from as little as 10 ng DNA/well LINE-1 probe used for DNA capture for better specificity Biologically relevant standards provided for improved quantification Active Motif’s Global […]
Histone Purification Microplate Kit
Histone purification system for high throughput applications Active Motif’s Histone Purification Microplate Kit enables purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material, and its microplate spin column format enables elution into a 96-well plate to easily transition into downstream high-throughput […]