The Tag-ChIP-IT® Kit offers a method to perform chromatin immunoprecipitation (ChIP) on transcription factor sequence variants, mutations and truncations without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag. Active Motif’s unique AM-tag sequence is specifically designed to work in ChIP. It has minimal […]
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Cas9 Monoclonal Antibody
Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is […]
Recombinant Nucleosomes
Whether you are performing drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications, substrate selection is critical. Nucleosomes offer the advantage of providing a more biologically relevant substrate when compared to histone proteins alone or synthetic peptides. To best mimic cellular biology with your in vitro assay, use Active Motif’s Recombinant Nucleosomes. […]
ChIP-Bis-Seq
Active Motif’s new ChIP-Bisulfite-Sequencing (ChIP-Bis-Seq) Kit offers a highly optimized and user-friendly method to directly interrogate both the chromatin and DNA methylation profile on the same sample within a single experiment. The kit combines a chromatin immunoprecipitation (ChIP) procedure for target-specific enrichment of protein-bound or modified chromatin with bisulfite conversion and sequencing (Bis-Seq) to provide […]
ChIP-exo Kit
Active Motif’s ChIP-exonuclease (ChIP-exo) Kit offers an optimized and streamlined protocol for high-resolution genome-wide mapping of transcription factor binding sites in the human and mouse genomes. The fragmentation step of traditional ChIP protocols can produce oversized DNA fragments that interact with more than just the binding site of interest. ChIP-exo is a modified ChIP-Seq approach […]