Perform ChIP on difficult-to-lyse PBMCs: Active Motif’s ChIP-IT® PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in […]

Perform ChIP from FFPE samples Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for techniques such as ChIP. Active Motif has leveraged its ChIP expertise to develop the first commercially available kits to enable ChIP from FFPE tissue for use in Next Generation […]

Don’t let some big corporation dictate your ChIP protocol! For researchers interested in streamlining their own workflow, Active Motif offers Protein G Agarose Columns. These are ready-to-use ChIP columns that contain the same high affinity protein G agarose beads included in the ChIP-IT® High Sensitivity Kit. The beads have been specifically engineered to eliminate non-specific […]

The combination of ChIP with genome-wide analysis using Next-Generation sequencing (ChIP-Seq) is a powerful approach that can provide insights into gene regulation by identifying global binding sites for proteins of interest. Active Motif’s ChIP-IT® ChIP-Seq Kit provides proven reagents, streamlined protocols and validation controls to give you confidence in successful ChIP-Seq using the Illumina®sequencing platforms. The […]