PfuTurbo Cx Hotstart DNA Polymerase Boosts Your BS-Seq PerformanceOctober 19, 2010Amplifying converted DNA is one of the trickiest steps in any Bisulfite conversion based protocol. A mistake here could stop your study cold if the amplification fails, or worse yet, you might be introducing errors that you won’t see until you’ve spent hours combing through useless sequencing data. Pfu-Turbo Cx Hotstart DNA Polymerase from Agilent’s Stratagene […]
Catch RNA and Protein in the Act with RNA ChIP ITOctober 1, 2010Long and short RNA have been frequenting the nucleus for years. Until now researchers have had plenty of good technical reasons to avoid this hot spot for RNA-protein interactions. Hey, we don’t blame you. ChIP can be tricky. RNA can be a little temperamental too. Fortunately, Active Motif took on both of these issues with […]
Put Your DNAm in a Bind with Clontech’s EpiXplore™ Methylated DNA Enrichment KitJuly 19, 2010Poor DNA methylation enrichment can really leave you in a tight spot when it comes to downstream analysis, so why not turn the tables and put your samples in a bind? Before you begin your next methyl-profiling experiment, make sure you have a solid methylated DNA enrichment kit, like the EpiXplore™ Kit from Clontech, handy […]
Digging Deeper with Invitrogen’s MethylMiner™ Methylated DNA Enrichment KitApril 15, 2010Methyl-binding proteins are rapidly becoming a preferred solution for researchers looking to boost the sensitivity of their DNA methylation analysis. Current approaches that use antibodies for DNA methylation enrichment have been useful to many, but alternative methods that use methylation binding proteins like the MBD2 in Invitrogen’s MethylMiner™ Kit grip methylated DNA tighter than skinny jeans on […]
Crush miRNA Function with miRZip anti-miRNA ConstructsApril 8, 2010The exact role miRNAs play in gene regulation may be an evolving story but that doesn’t mean you can’t pin down miRNA function in your model system. miRZip anti-miRNA vectors are a new breed of miRNA inhibitors that allow you to permanently knockdown miRNA function so you can analyze changes in phenotype more easily. Equipped with an H1 promoter, […]
miRNA Functional Analysis Unleashed with Ambion® Anti-miR™ miRNA Inhibitors and Pre-miR™ miRNA PrecursorsAugust 28, 2009So you’ve just wrapped up a bunch of miRNA profiling experiments and now it’s time to get functional. Like any researcher studying miRNA you probably lie awake at night asking yourself: What is it targeting? With what pathways is it involved? What happens when there is too much, or not enough of it? To help […]
Bulletproof Your Bisulfite Conversion with Zymo’s EZ DNA Methylation-Startup™ KitJune 29, 2009Instant Methylation Data: Just Add DNA With new systems like Zymo’s EZ DNA Methylation-Startup™Kit, it’s easier than ever to get up and converting in no time. So if your lab has been plugging away looking at chromatin modifications or miRNA profiles and want to cross over and see how DNA methylation is involved, you don’t have […]
RIP Into RISC Proteins with System Biosciences’ miR-SNaREsApril 14, 2009Small Non-coding RNA Enrichment Can Be RISCy Things can get complicated when you are looking for low copy miRNAs, validating miRNA targets or trying to figure out all of the players in RISC complexes. SBI’s miR-SNaREs™, enable you to lasso those answers easily. The system uses Lentivectors to overexpress different flavors of epitope tagged microRNA processing […]
miRNA Promoter Analysis with Custom ChIP-Chip ArraysApril 11, 2009Last time we checked there weren’t any miRNA Promoter Arrays out there in the market and even if there were, they’d likely be out of date, so if you’re ready to roll up your lab sleeves and dive into the transcriptional regulation of miRNAs, you’ll want to check into custom array designs like Agilent’s eArray. These custom, ink […]
