Features

Features

EpiGenie Features call out new Methods, provide Insights into emerging trends, or sometimes just share technical tips from the experts in our community.

Search Articles

Expert Insight: 5-hmC Analysis Methods

August 5, 2010

Last year’s discovery of 5-hydroxymethylcytosine (5-hmC) caused some epigenetics researchers to suffer a crisis of confidence, provoking many hours of soul-searching questions such as “are my methylation data really accurate?” and “what if what I thought was gene silencing is really activation?” Although current estimates place 5-hmC levels at only a fraction of the 5-mC […]

Triplex Oligos Enable Targeted DNA Methylation

July 8, 2010

There’s a new tool out there, and it’s made for all of those researchers out there who have ever pointed to a bit of genomic sequence and wondered, “what would happen if that promoter CpG was methylated right there?” A new technique detailed in the latest issue of Bioconjugate Chemistry can help answer questions like […]

Nucleosome Dynamics: CATCH-IT if You Can

June 9, 2010

Since our interview last year with Steve Henikoff, in which we discussed his Roadmap Epigenomics R21 grant, we’ve been itching to learn all the details of his new method for studying nucleosome turnover. On May 28, our wait was finally over with the debut of the CATCH-IT (covalent attachment of tags to capture histones and […]

CpG Islands Tackle Chromatin Remodeling Project

April 28, 2010

Researchers have known about CpG Islands (CGIs) for decades, but there are still more questions than answers when it comes to this heavily studied genomic real-estate. Hypermethylated CGIs seem to get a lot of attention for their apparent involvement in transcriptional silencing, but most CGIs stay unmethylated. So what’s going on in these unmethylated CGIs? […]

ChIP-Sequencing Tips for Small Samples

April 28, 2010

ChIP sequencing (ChIP-Seq) has revolutionized the way researchers can study protein-DNA interactions, enabling genome wide protein binding profiling in one experiment, rather than looking one locus at a time. Not satisfied with pushing just one envelope, today’s researchers are now moving towards samples that are very limited. Fortunately, ChIP methods have aged finer than a […]

Hydroxymethylcytosine Shakes Up the Epigenetics Community

April 15, 2010

5-hydroxymethylcytosine’s (5-hmC) discovery last year in mammalian DNA (Kriaucionis & Heintz, Science, 2009. and Tahiliani et al., Science, 2009), left the epigenetics community more shaken than a 007 martini. Although its broader role (if any) in epigenetics remains a mystery today, early evidence suggests a few putative mechanisms that could have big implications: Conversion of […]

Decoding Autism’s Epigenetic Riddle

April 14, 2010

Autism is a developmental disorder that’s shrouded in mystery. Its causes are unknown and hotly debated, but some recent detective work hints that through changes in miRNA expression and DNA methylation (DNAm) patterns, there is an epigenetic link. Autism often brings to mind Dustin Hoffman’s character in Rain Man, who was deeply focused on only […]

Single Tube DNAm Analysis. Better Than Sliced Bread?

April 6, 2010

Tired of performing DNA extractions, bisulfite conversions, and PCR in three separate reactions? Is the sweet smell of phenol not helping you out with the opposite sex? Then, you’ll love DNA methylation analysis using methylation on beads (MOB)! Although it may sound like the stuff of infomercials, a team at Johns Hopkins University really found […]

Breaking Down miRNA Microarrays

April 6, 2010

As analysis of miRNAs becomes increasingly popular, the resulting flood of microarray products out there for miRNA profiling is enough to make your head spin. So how do you decide which ones to use? If you’ve been stressing over that question, you’ll be happy to know that some good folks at Cambridge Research Institute and […]

Non-Coding RNAs in Epigenetics

March 30, 2010

We interviewed John Mattick a few years back as we were getting ready to launch EpiGenie, but couldn’t manage to get the transcripts all edited and ready for show time. So, when we ran accross this interview in what was Invitrogen’s Quest magazine we were excited, then bummed because we didn’t run it first, then […]

Pages: 1234567891011121314151617181920212223242526272829303132

TAP

Features

Featured Articles

Search Articles