RIP-Seq Shows miRNA Expression and Function Not Always a MatchJune 20, 2013There is a saying that, “each microRNA can have hundreds of target mRNAs, and each mRNA can be targeted by hundreds of miRNAs”. If that’s true, then how the heck are we supposed to know what’s going on in that crazy transcriptome? What does it mean when a miRNA and predicted target don’t explain the […]
Application Note: Automated Bisulfite ConversionJune 14, 2013Unlike more mainstream assays, bisulfite conversion approaches have been dependent on manual manipulation of spin plates and columns or have been of limited throughput. By adapting the clean-up of bisulfite converted DNA to a magnetic bead based procedure and coupling it to the Freedom EVO® platform, Zymo Research has opened the door to high-throughput bisulfite […]
Optical Detection Adds Color to 5hmC AnalysisJune 11, 2013A talented crew of researchers led by Dr. Yuval Ebenstein (Tel-Aviv University, Israel) recently pioneered a technique that gives anyone the ability to assay 5hmc “nanodrop” style. In their paper, they presented proof-of-principle experimentation. They labeled DNA and then sandwiched it between two glass slides to stretch it.. The DNA was then imaged on a […]
The Consequences of RNAs Alternative Polyadenylation LifestyleJune 7, 2013First it started enjoying the ‘occasional’ methylation. Then it crossed the LINE when it ‘borrowed’ its good buddy’s machinery. And now it seems like RNA just can’t stop. It turns out that just like all those hipsters, splicing isn’t the only one to enjoy an ‘alternative’ lifestyle. Researchers from UCLA have shown that polyadenylation wants […]
Epigenetics: Getting to Know the Readers and the Writers with Dr. Jerry WorkmanMay 31, 2013Dr. Jerry Workman shares some of his thoughts on the next steps in digging into biochemistry of epigenetic mechanisms to understand what’s reading and what’s writing. Epigenetics: Getting to Know the Readers and the Writers I think the most exciting aspect about epigenetics at this time is the potential it provides for some fantastic […]
