3.7M CpGs Await with Improved Methyl-Seq Target EnrichmentMarch 3, 2012March 3, 2012 Instead of sequencing (and paying for, managing, storing, and analyzing) everything, target enrichment blazes through samples like a combine, harvesting for regions of interest. Even as sequencing costs drop towards that “$1,000 genome” level, enriching for select targets makes a lot of sense, especially if you only want to look at a […]
Epigenetics at the Intersection of Environment and SelectionFebruary 28, 2012One of the most fascinating things about epigenetics is the implication it has for inheritance and evolution. Theories and hypotheses are everywhere, of course, but until Sir Richard Branson launches a Virgin Time Travel business, actually measuring the changes from thousands of years of natural selection will remain a pretty tall order. Two recent papers […]
Epigenetic Regulation of Gene ExpressionFebruary 25, 2012EpiGenie recently reviewed the epigenetic text book RNA and the Regulation of Gene Expression. Here we give you a small taste of the content found inside with a summary of one of the chapters: Epigenetic Regulation of Gene Expression by Kevin Morris This chapter outlines the fundamental aspects of epigenetic regulation as it relates to […]
Small RNA-mediated Gene ActivationFebruary 25, 2012EpiGenie recently reviewed the epigenetic text RNA and the Regulation of Gene Expression by Kevin Morris. To give you a small taste of the material covered in the book, here is a summary of one of the chapters: Small RNA-mediated Gene Activation by Long-Cheng Li Noncoding RNAs (ncRNA) which, by definition, do not code for […]
Getting your Fix: Optimizing Chromatin Fixation for ChIP AnalysisFebruary 23, 2012Various ChIP methods are filled with all sorts of secret tricks that don’t seem to make it into the textbooks. This is especially true of the chromatin fixation step, where loads of variables make it difficult to pin down the exact conditions you need. Instead of learning the hard way, through failed experiments or apprenticing […]
