ChromID Helps Us Understand How Epigenetic Pots Find Their Protein LidsMarch 16, 2020The old saying “every pot will find its lid” suggests that a match exists for everyone looking for love, you just have to get out there and find your perfect partner! Online-dating may help scientists to find their significant other from the bench side; however, current laboratory approaches for the identification of protein-binding partners for […]
EpiGenie Guide: Chromatin ShearingMarch 6, 2020ChIP-Seq has transformed the field of epigenetics, providing unprecedented resolution and coverage for surveying protein- DNA interactions. Powerful, high-throughput sequencing is credited with most of the benefits, but there’s a lot more that goes into ChIP-Seq than sequencing. Chromatin shearing is one of the first critical steps in the ChIP-Seq workflow. Depending on your approach, […]
dCas9-ROS1 Cuts Out the Methylation Middlemen in Targeted DNA DemethylationMarch 3, 2020As the saying goes, the shortest path from A to B is a straight line and if you’re studying DNA demethylation, you know just how complicated things can get along the road from methylated to unmethylated cytosines. Mammals use Ten-Eleven Translocation proteins (TET) to oxidize 5mC, but the process creates pit stops that may be […]
Epigenetic Study Suggests that the Safety of Vaping is Running on FumesMarch 2, 2020With as many flavors as there are epigenetic modifications, vaping has arrived on the scene as a safer alternative to the smoking of cigarettes and a means to give up a rather unhealthy habit. However, the rise in vaping-related lung injuries and the “unforeseen” massive uptake of vaping by teenagers have prompted scientists to undertake […]
Inactive X Chromosomes SPENd Time Tucked Under the Epigenetic CoversFebruary 25, 2020When it’s the dead of winter, and the world outside is frozen solid, there’s no better place to be than under a mountain of warm blankets, like an inactivated X chromosome under a cloud of Xist long-noncoding RNA. Although we’ve known for a while that Xist is necessary for X chromosome inactivation (XCI), the mechanisms […]
