Webinar: An Engineered Synergistic CRISPR/Cas9 Activator Complex for Genome-wide Transcriptional ControlMarch 4, 2015Presenter: Silvana Konermann, Graduate Student in Dr Feng Zhang’s Lab at Massachusetts Institute of Technology Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. The ease and scalability of the CRISPR-Cas9 system potentially enables systematic, genome-scale perturbation, but the magnitude of transcriptional up-regulation achieved by the first generation of Cas9 transcriptional […]
Epigenetic Sequencing Woes Overcome by Services Know-howMarch 1, 2015Sequencing technologies have created a dizzying number of opportunities for epigenetic researchers to explore questions that couldn’t be tackled before. Despite the immense power that sequencing delivers, though, the whole process still isn’t quite as simple as pushing a big red button, and watching the groundbreaking discoveries roll in. Even as sequencing becomes faster, easier, […]
Express Yourself with Light-activatable CRISPR-Cas9February 26, 2015Guess what? It seems that blue light has a lot more to offer than just helping with your winter time blues. It could also be just what your transcriptional activation system needs. Synthetic biology has a lot to offer omics beyond genome editing and recent work from multiple groups is putting Cas9 in a different spotlight. CRISPR/Cas9 […]
m6Ajor New Function for MicroRNAs in RNA MethylationFebruary 20, 2015It’s no secret that microRNAs are pretty busy molecules. A single microRNA can regulate the expression of several, maybe even hundreds, of transcripts. If that wasn’t enough, researchers at the Chinese Academy of Sciences, Beijing now add another function to this packed list of duties: the control of m6A RNA methylation, which is emerging as an […]
Getting started with Chromatin Conformation Capture (3C)February 15, 2015The concept of chromatin contact mapping, or determining the three-dimensional structure conformation and interactions of chromatin domains, is now a reality because of Chromatin Conformation Capture (3C) and subsequent methods born out of that approach. Several new 3C-based techniques have emerged, each with particular strengths and applications, but the sheer variety creates a challenge when […]
