DNA Methylation Map Based on Data from 82 Human Cell Lines and TissuesJanuary 21, 2013Christmas has come and gone, but the ENCODE Project is the gift that keeps on giving. Using samples and data from ENCODE (in combo with their own data), researchers have come up with a single-base resolution map of DNA methylation across 82 human cell lines and tissues, including many cancers. With the ever increasing DNA methylation data […]
Visualize Histone Modifications in Single Cells? Yes You Can.January 18, 2013ChIP has been a dear friend to researchers studying histone modifications for years. But, just like most friends, it has its imperfections too, particularly when it comes to sensitivity. On its best day ChIP is about as sensitive as Mr. Trump and just like The Donald, it can be temperamental. So we were excited to […]
mRNA Alternative Polyadenylation Lifestyles Comes into FocusJanuary 17, 2013As the only epigenetics media group based out of southern California, we’ve learned to be very accepting of alternative lifestyles, which is why this method caught our eye. mRNA are a diverse bunch, with all kinds of alternative polyadenylation that can affect their cellular localization and stability. RNA Seq has enabled unprecedented glimpses into the transcriptome, but […]
Making Sense of the Grade: ChIP Validation TerminologyJanuary 17, 2013Antibody vendors have been hard at work to offer antibodies that are suitable for applications like ChIP. Usually these antibodies have been used successfully in peer-reviewed publications. In many cases they’ve been validated internally by an R&D team, and in some cases, each lot may be validated to work in ChIP applications. The tricky part […]
MeCP2 Puts 5hmC in a Bind in Active Neuronal GenesJanuary 7, 2013A good actor can play very different roles and still make you believe in the character. For example, who knew Wolverine could emote and sing, like Hugh Jackman does in Les Misérables? Just the same, it turns out that MeCP2, a protein thought to repress transcription by binding to 5mC (5-methylcytosine, actually seems to encourage […]
