DNMT1 Depletion Helps Hunt for Heritable MethylationFebruary 5, 2015Sometimes it takes a special kind of guy to get the job done and when the job is to keep imprinted genes differentially methylated, that guy is DNMT1. An international group of researchers led by Jacquetta Trasler in Canada set out to exploit this fact in order to identify yet unknown imprinted genomic sequences. Several […]
Neurodevelopment and Methylomic Trajectories: Sex and DiseaseFebruary 4, 2015If variety is the spice to life than the brain must be the spiciest organ of them all, with DNA methylation as its rooster sauce (which comes with its own embedded sodium bisuflite conversion kit). By studying the delicious layers of DNA methylation variation we’ve seen strange parental expression biases, methylation differences between discordant twins, and how the dynamics […]
A Good Friend is Forever: DNMTs and Histone ComplexesJanuary 26, 2015It’s not always easy to find good friends, but recent work has found that the de novo DNA methyltransferases DNMT3A2 and DNMT3B1 are far from socially awkward. Baubec et al. from the Swiss team lead by Dirk Schübeler uncovered some companions of these DNMTs by profiling their binding in embryonic stem cells (ESCs) through streptavidin […]
Abcam’s Bisulfite-Seq High Sensitivity Kit Streamlines 5mC DetectionJanuary 26, 2015The new Bisulfite-Seq High Sensitivity Kit from the creative crew at Abcam, is a 2-in-1 package that weaves bisulfite conversion, and Illumina® sequencing library prep into the same process. This single kit can take you from sample DNA, to firing up a Bisulfite Sequencing (BS) run that same day. There’s No Such Thing as Too […]
Active Demethylation: TET Enzymes, 5hmC, and CompanyJanuary 20, 20155hmC was originally discovered way back in the 50’s in bacteriophages and was first observed in mammals in the 70’s, but research into this was set back when these studies failed to replicate. And going back to the future, in 2009 two independent research groups, publishing on the same pages in science, found that this “unknown […]