New and Improved: Multiplexed Reduced Representation Bisulfite Sequencing (RRBS)December 7, 2012These days, we’re all about multi-tasking—talking on our cell phones while placing our cappuccino order at Starbucks and tweeting about it all on our tablets. In that same vein, researchers report an optimized protocol for multi-tasking (well, technically “multiplexing”) reduced representation bisulfite sequencing (RRBS) for ramped-up DNA methylation data. RRBS is great, churning out methylation […]
The Daily Rhythms of Transcription and the EpigenomeDecember 7, 2012We all have a daily routine. For much of the EpiGenie office staff, that includes getting out of bed, brushing our teeth, going to work, grabbing a coffee, clocking a few hours on Facebook and complaining that there aren’t enough hours in the day. Now, researchers say that transcription and epigenetic modifications in mouse liver […]
Epigenetic Drift in Stem CellsNovember 28, 2012Living for a while in a new environment can have a big effect on how you dress, talk, and act. Just listen to Madonna’s British accent. Likewise, taking bone marrow mesenchymal stem cells (BM-MSCs) out of the body and into the in vitro cell-culture environment can change them—but not necessarily for the better. In order […]
Lentiviral Vectors Can Cause Epigenetic ModificationsNovember 20, 2012Stem cell programming has been buzzing the last few years and with recent work suggesting that epigenetic and ncRNA factors are manipulative enough to star in TV drama, we expect the excitement to continue. But according to a recent report, researchers may need to keep close tabs on how the very methods they use to […]
EpiGenie 5hmC Technology GuideOctober 31, 2012A couple of years ago, researchers keen to analyze 5-hmC had a couple of options, but those early approaches couldn’t quite satisfy all of their increasing demands. Fast-forward a bit and it’s a very different story. Today there’s a plethora of options, each with its own strengths and weaknesses. As a result, one of the […]
