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Protein Methylation: The Other Epigenetic Regulator

August 13, 2010

While epigenetic mechanisms like DNA methylation and chromatin remodeling get most of the attention, more is being learned everyday about other important mechanisms that are just now starting to get their share of the limelight. In this article contributed by John Aletta at CH3 Biosystems, he explains how protein arginine methylation is also a key […]

Expert Insight: 5-hmC Analysis Methods

August 5, 2010

Last year’s discovery of 5-hydroxymethylcytosine (5-hmC) caused some epigenetics researchers to suffer a crisis of confidence, provoking many hours of soul-searching questions such as “are my methylation data really accurate?” and “what if what I thought was gene silencing is really activation?” Although current estimates place 5-hmC levels at only a fraction of the 5-mC […]

FFPE: Friend or Foe to Chromatin Analysis?

August 4, 2010

It Seems like a no-brainer that chopping up proteins and denaturing DNA would have a dramatic effect on chromatin structure. Since so many cancer diagnoses, developmental studies, and examinations of chromatin structure in general rely on slices of wax-embedded tissue blocks, you’d think someone would take a comprehensive look at the effects of prepping those […]

iPS Cells Can’t Escape Their miRNA Pasts

August 4, 2010

Even though the second season of The Jersey Shore takes place in Miami, the cast remain true to their ‘guido’ roots. I guess sometimes you just can’t change who you really are. The same is true for microRNA expression profiles of iPS cells. A recent review (Lakshmipathy et.al. 2010) from Ron Hart’s group at the […]

Uncovering 5hmC’s Link to Stem Cell Self-Renewal

August 3, 2010

The search is on to figure out a role for the recently discovered 5-hydroxymethylcytosine (5hmC) base. While it’s still up in the air if 5hmC will play a main character or just be cast as an extra , this new study in Nature ties the mysterious modification to a demethylation pathway, regulated by Tet proteins, […]

EpiGenie 2010 Mid-Year Editor’s Favorites: Non-Coding RNA

July 21, 2010

We’re halfway through 2010, so we’ve decided to take a look back at some of the best epigenetics publications we’ve covered so far this year. Here are some of our favorite Non-Coding RNA headlines. miRNA Decoys Psuedogenes finally get some love. Long considered to just be wasted genomic space, it turns out that they do […]

EpiGenie 2010 Mid-Year Editor’s Favorites: DNA Methylation

July 21, 2010

We’re halfway through 2010, so we’ve decided to take a look back at some of the best epigenetics publications we’ve covered so far this year. Here are some of our favorite DNA Methylation headlines. CpG-SNPs If your methylation profiles have been looking a little fuzzy, it could be CpG-SNPs are to blame. CpG-SNPs where found […]

EpiGenie 2010 Mid-Year Editor’s Favorites: Chromatin

July 21, 2010

We’re halfway through 2010, so we’ve decided to take a look back at some of the best epigenetics publications we’ve covered so far this year. Here are some of our favorite Chromatin headlines. Histone Mods and Alternative Splicing This publication might have popped up in the mainest of mainstream journals, but the focus is all […]

Genes Weigh Their Alternative Promoter Options

July 8, 2010

It’s always nice to have options, but nowadays there are often so many choices that you can drive yourself crazy making a decision, just ask LeBron James. A recent Nature paper shows that even genes need a little input from DNA methylation when they consider using alternative gene promoters. Joseph Costello at UCSF and colleagues […]

Triplex Oligos Enable Targeted DNA Methylation

July 8, 2010

There’s a new tool out there, and it’s made for all of those researchers out there who have ever pointed to a bit of genomic sequence and wondered, “what would happen if that promoter CpG was methylated right there?” A new technique detailed in the latest issue of Bioconjugate Chemistry can help answer questions like […]

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