Editor in Chief: TALEN, CRISPR and the iPSC GenomeAugust 2, 2014Undeniably, the future of therapeutics is moving away from panacea miracle drugs and gravitating towards personalized treatments using gene therapy. Furthermore, the discovery of iPSCs verified the idea that individuals could have their own stem cell line available for therapeutic purposes—therapies that are made possible by editing the genome of iPSCs. Transcription activator-like effector (TALEN) and […]
CRISPR Paves the Way for Large Fragment Genome EditingAugust 1, 2014Traditional large genome editing techniques employ indirect cloning of large genomes into bacterial artificial chromosome (BAC) plasmid. This technique is rather difficult and protracted. Thus, bypassing the BAC plasmid stage in large genome editing would be a big win for the application. The CRISPR-cas9 system, first discovered in Streptococcus pyogenes, is an important tool for […]
Hold Your ‘breadth’ – H3K4me3 Domain Size MattersAugust 1, 2014Apple may have finally thrown the gloves off in the race for larger screens, but it appears that this is old news for cells, who have been elevating the status of important genes for years by marking them with large billboards of H3K4 tri-methylation. These genes then get the VIP treatment from cellular machinery ensuring “enhanced […]
Webinar: Using CRISPR/Cas9 Technology for Editing Genes in Haematopoietic Cells and MiceJuly 29, 2014Presented by: Dr Marco Herold, Laboratory Head, Molecular Genetics of Cancer Division, The Walter & Eliza Hall Institute of Medical Research Abstract: The CRISPR/Cas9 technology provides an easy and rapid way to edit genes in vitro and in vivo. Initial experimental strategies utilized a transient transfection approach to modulate genes in vitro, but this proved […]
TAL Effectors Dig the Long Distance ThingJuly 25, 2014If you think the subway is crowded you haven’t seen what a TAL Effector (TALE) has to put up with when getting to work. In a new study involving the production of more than 200 TALE modulators, researches at Beijing University found the long sought explanation of why some TALEs effectively modulate transcription while others do not. […]
