Catch RNA and Protein in the Act with RNA ChIP ITOctober 1, 2010Long and short RNA have been frequenting the nucleus for years. Until now researchers have had plenty of good technical reasons to avoid this hot spot for RNA-protein interactions. Hey, we don’t blame you. ChIP can be tricky. RNA can be a little temperamental too. Fortunately, Active Motif took on both of these issues with […]
O-allylhydroxylamine Threatens Bisulfite’s Gold Standard StatusSeptember 23, 2010Let’s get ready to rumble! A new reagent, O-allylhydroxylamine, has entered the ring to challenge bisulfite conversion as the undisputed champion of DNA methylation analysis. In the latest issue of Nucleic Acids Research, chemists from Ludwig-Maximillians University in Germany introduced a new chemical reagent that could be a game-changer for distinguishing between cytosine (C) and […]
Heterogeneous DNA Methylation and Biomarkers: It’s ComplicatedSeptember 23, 2010The state of the economy, global climate change, DNA methylation…These are all complicated issues. Well, we found one well-written review article by Thomas Mikeska, Ida Candiloro, and Alexander Dobrovic at the Peter MacCallum Cancer Centre and the University of Melbourne in Australia that tries to put one of those topics, DNA methylation in perspective, at […]
miRNAs Go with the FlowSeptember 23, 2010Many of us dread the inevitable needle prick at the doctor’s office to draw blood, not to mention even more invasive diagnostic tests (colonoscopy, anyone?). Wouldn’t it be easier if we could just spit or pee in a cup, and from the spectrum of miRNAs in the fluid, our doctor could tell us whether or […]
DNA Methylation Holds Steady in Body Mass IndexSeptember 17, 2010Mules are well known for being stubborn creatures, but they aren’t alone. It seems that resistance to change is something they may have in common with body weight and certain stretches of your epigenome. Andrew Feinberg and M. Daniele Fallin at Johns Hopkins University led several teams in a study that shows, for the first […]
