The Daily Rhythms of Transcription and the EpigenomeDecember 7, 2012We all have a daily routine. For much of the EpiGenie office staff, that includes getting out of bed, brushing our teeth, going to work, grabbing a coffee, clocking a few hours on Facebook and complaining that there aren’t enough hours in the day. Now, researchers say that transcription and epigenetic modifications in mouse liver […]
Low Cell ChIP-SeqNovember 28, 2012Chilly, wintry temps have us longing for palm trees, sandy beaches, and the limbo call of “How low can you go?” Researchers in Norway and Germany have the answer—and a new ChIP-seq method that allows them to analyze genome-wide DNA-protein interaction data for samples that have very few cells, without culturing (which could alter epigenetic […]
Epigenetic Drift in Stem CellsNovember 28, 2012Living for a while in a new environment can have a big effect on how you dress, talk, and act. Just listen to Madonna’s British accent. Likewise, taking bone marrow mesenchymal stem cells (BM-MSCs) out of the body and into the in vitro cell-culture environment can change them—but not necessarily for the better. In order […]
MNase-Seq Produces High Resolution Nucleosome Positioning MapsNovember 27, 2012Nucleosomes don’t have GPS, so how do they know where to go? Are they haphazardly strewn across the genome or is there some logic to it? Researchers now say they have some answers. They found that DNA sequence and DNA-binding proteins play roles in determining where nucleosomes end up, and that most nucleosomes are more […]
One Step ChIP with Abcam’s EpiSeeker ChIP KitNovember 20, 2012Antibody companies like Abcam run a ton of ChIP experiments, so they’re all too familiar with the amount of variability that can lurk in every step of the ChIP protocol. So what’s the safest way to reduce variability and get consistent results? Reduce the steps of course; and that’s exactly what the EpiSeeker ChIP Kit […]
