ChIP-Seq Libraries Made Easy with NEBNext® ReagentsFebruary 1, 2011A wealth of answers exist within the world’s libraries. Shouldn’t the same be true for your ChIP-Seq library? Sequencing has catapulted the output of ChIP analyses to levels most researchers wouldn’t have dreamed of just a few short years ago, but the quality of all of that output depends on a number of factors, including […]
Take a Bite Out of Cancer with the Epigenetics DietFebruary 1, 2011What’s your idea of a perfect meal? Whether you’re into the In-N-Out burger, fries, and milkshake trifecta, or you’re more of a “cleanse” type, the epigenetics enthusiast in you will be interested in a recent review by Trygve Tollefsbol and colleagues that reveals that certain foods contain ingredients that may prevent and treat cancer. Although […]
First Exon Methylation Gets Last Word On SilencingJanuary 31, 2011Arguments are often won by whoever gets in the last word, and while CpG Islands have made the most noise in the debate over how DNA methylation controls transcription, other gene regions like exons are now chiming in too. The classic model where methylated CpG Islands in promoters shut down transcription of a gene has […]
Single Cell Methylation Analysis: Brought to You by RMSAJanuary 30, 2011As DNA methylation keeps popping up in the discussion of more and more diseases, it’s clear that current analysis techniques can only give us part of the big picture. To help focus on other parts of that picture, a new method has been devised that zooms in on the methylation status of single cells. The […]
Ta-da! Blood Cells Transform into iPSCsJanuary 20, 2011Turning a cell into an induced pluripotent stem cell (iPSC) is tricky. It turns out that blood cells have an edge—they already epigenetically resemble iPSCs. When U.S. and Chinese researchers poked around the DNA of human CD34+ cells from umbilical cord blood and adult blood, they found that the promoters’ methylation patterns looked a heck […]