oxBS-Seq Breaks Through 5hmC Sequencing BarrierApril 26, 20125-Hydroxymethylcytosine (5hmC) has always been a bear to sequence, mostly because it’s virtually indistinguishable from its close relative, 5-methylcytosine (5mC) using classic bisulfite sequencing techniques. Now a new sample preparation method has hit the scene that can discriminate between cytosine, 5mC and 5hmC at single base resolution, called oxidative bisulfite sequencing (oxBS-Seq) that essentially smashes […]
Sequencing DNA Methylation Past, Present, Future with Dr. Wolf ReikApril 26, 2012Dr. Wolf Reik discusses the challenges in DNA methylation sequencing and the next big swings in epigenetic modification sequencing and discovery. This interview took place at the Keystone Symposia’s Epigenomics and Chromatin Dynamics joint meeting in January, 2012. This video was brought to you by epigenetics supporters at EMD Millipore. Sequencing DNA Methylation […]
Argonaute AGO2 Gives miRNAs a Little Trim Around the EdgesApril 17, 2012Isn’t there always one person in a group of friends who’s a little weird or different? It turns out that AGO2, is a little different from its Argonaute paralogs, AGO1, 3, and 4. Researchers in Singapore are reporting that unlike other Argonautes, oddball AGO2 trims mammalian miRNAs, changing their functions during development. The team knew […]
miRNAs Decide Which Honey Bees Do Which Honey-Do’sApril 17, 2012It seems nobody has been busier than the honey bee lately when it comes to being a model epigenetic organism. (Watch your back c. elegans!) Over the last year or so, we’ve seen how HDAC inhibitors affect the bee caste system, as well as how social cues alter DNA methylation patterns in the brains of […]
Active Motif MeDIP-Seq Services Will Put You On the Right MethylPath™April 17, 2012Let’s say you want to get the ball rolling for studies on a new disease. You know you want to search for any important changes in methylation patterns; but with a whole genome worth of ground to cover, how do you decide where to look first? Active Motif now offers the MethylPath™ MeDIP-Seq Service that […]