One Step ChIP with Abcam’s EpiSeeker ChIP KitNovember 20, 2012Antibody companies like Abcam run a ton of ChIP experiments, so they’re all too familiar with the amount of variability that can lurk in every step of the ChIP protocol. So what’s the safest way to reduce variability and get consistent results? Reduce the steps of course; and that’s exactly what the EpiSeeker ChIP Kit […]
Lentiviral Vectors Can Cause Epigenetic ModificationsNovember 20, 2012Stem cell programming has been buzzing the last few years and with recent work suggesting that epigenetic and ncRNA factors are manipulative enough to star in TV drama, we expect the excitement to continue. But according to a recent report, researchers may need to keep close tabs on how the very methods they use to […]
miR 126 Sponges: The Gift for the Stem Cell Researcher that has EverythingNovember 20, 2012The gift giving started bit early this year for the stem cell community as an international team of researchers shared key findings that could transform clinical applications of human blood stem cells. They wrapped up miR-126 binding sites in lentiviral vectors and put a serious smack down on miR-126 activity in hematopoietic stem cells (HSCs). […]
EpiGenie 5hmC Technology GuideOctober 31, 2012A couple of years ago, researchers keen to analyze 5-hmC had a couple of options, but those early approaches couldn’t quite satisfy all of their increasing demands. Fast-forward a bit and it’s a very different story. Today there’s a plethora of options, each with its own strengths and weaknesses. As a result, one of the […]
This Stem Cell Reprogramming is Made Possible with Generous Support from Histone H3.3October 30, 2012Some people dress up their pets in clothes so that they will look more like their human owners. Turns out, a similar thing happens in the nuclear transfer process to make stem cells. Jerome Jullien and colleagues in 2012 Nobel laureate John B. Gurdon’s lab (along with a number of other great researchers in France […]
