DNA Methylation qPCR RemixedApril 5, 2011qPCR is used daily to validate array and sequencing results, but qPCR approaches have yet to dominate DNA methylation analysis the same way they have with other applications like gene expression analysis or genotyping. Things are a little bit more complicated when it comes to the epigenetic mods, but that hasn’t stopped hungry researchers from […]
Chromatin States Get On the MapMarch 30, 2011Deciphering chromatin status across an entire genome must seem like trying to navigate through an Amazon rain forest without a guide. Luckily, new work just published begins the process of creating chromatin state roadmaps to help point epigenetics researchers down the right path. To make that journey a little smoother, a research group from MIT […]
Pull Down a Winner with Abcam Antibody PanelsMarch 29, 2011It’s a big step. It’s stressful. Deciding which antibody will be “the one” in your ChIP applications isn’t easy, but you can relax a bit now as our good friends at Abcam must have sensed a touch of that antibody commitment-phobia within the research ranks, as they’ve just launched Abcam Antibody Panels; a way to let […]
miRNA Mimics Take Imperfect Aim at Gene PromotersMarch 29, 2011Sometimes a perfect match isn’t all it’s cracked up to be – just look at the Hollywood divorce rate. Whether you’re talking relationships or ncRNAs, a few mismatches between partners can still lead to a successful outcome. That seems to be the case for small RNAs that recognize gene promoter regions, as revealed by researchers […]
Making The ChIP Antibody GradesMarch 29, 2011Back in the 2000’s most researchers spent their post-ChIP analysis looking at a gel or a PCR curve. Now, they spend their analysis time combing through hard drives of sequencing data. A lot has changed in the world of location analysis, but the need for good antibodies remains the same; which is probably why we […]