Compound Effects on DNA Methylation: Prenatal Alcohol Exposure and Early Life StressMarch 18, 2020While some compound effects, like interest, are desired, there are others we should avoid. While we know that stress and alcohol have adverse effects on pregnancy that can be reflected epigenetically, it turns out that the combination of the two can have distinct detrimental effects. Previously, the lab of Shiva Singh at Western University (Ontario, […]
Not Aging Gracefully? Two Epigenetic Regulators May be to BlameMarch 16, 2020In the last 50 years, there has been a ~20% increase in global life expectancy but interestingly, there has not been a corresponding increase in the number of healthy years lived. Lubin Jiang and Shi-Qing Cai at the Chinese Academy of Sciences (Shanghai, China) sought to understand that disparity by examining the genetic factors involved […]
ChromID Helps Us Understand How Epigenetic Pots Find Their Protein LidsMarch 16, 2020The old saying “every pot will find its lid” suggests that a match exists for everyone looking for love, you just have to get out there and find your perfect partner! Online-dating may help scientists to find their significant other from the bench side; however, current laboratory approaches for the identification of protein-binding partners for […]
EpiGenie Guide: Chromatin ShearingMarch 6, 2020ChIP-Seq has transformed the field of epigenetics, providing unprecedented resolution and coverage for surveying protein- DNA interactions. Powerful, high-throughput sequencing is credited with most of the benefits, but there’s a lot more that goes into ChIP-Seq than sequencing. Chromatin shearing is one of the first critical steps in the ChIP-Seq workflow. Depending on your approach, […]
dCas9-ROS1 Cuts Out the Methylation Middlemen in Targeted DNA DemethylationMarch 3, 2020As the saying goes, the shortest path from A to B is a straight line and if you’re studying DNA demethylation, you know just how complicated things can get along the road from methylated to unmethylated cytosines. Mammals use Ten-Eleven Translocation proteins (TET) to oxidize 5mC, but the process creates pit stops that may be […]
